Can I take a tax deduction for my 401k Contribution on my tax return?

You can't take a tax deduction on your tax return for your 401k contribution because it has not been included in your taxable income. Your 401k contribution is not included as taxable income on your Form W-2 so you don't pay tax on it on your tax return.
 Related tax information about 401k contribution
Itemized Tax Deductions
IRS publications about 401k contribution:
For more information, refer to Tax Topic 424, 401(k) Plans, IRS Publication 575, Pension and Annuity Income (Including Simplified General Rule), or IRS Publication 560, Retirement Plans for the Self-Employed. Also see IRS Publication 17, Your Federal Income Tax.  
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For further information regarding IRS rules and regulations and your particular tax or IRS situation you should consult with a Certified Public Accountant, Enrolled Agent, Attorney, or other tax advisor. 
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